DETECTION OF APRICOT LATENT VIRUS TO PEACH IN CONSTANTA AREA

Authors

  • Catița Plopa Research Institute for Fruit Growing Pitești, Mărului Street, no. 402, 117450, Argeș; Research Station for Fruit Growing Constanța, no. 25 Pepinierei Street, Valu lui Traian, Constanța Author
  • Adina Floricica Iancu Research Institute for Fruit Growing Pitești, Mărului Street, no. 402, 117450, Argeș; Research Station for Fruit Growing Constanța, no. 25 Pepinierei Street, Valu lui Traian, Constanța Author
  • Corina Gavăt Research Institute for Fruit Growing Pitești, Mărului Street, no. 402, 117450, Argeș; Research Station for Fruit Growing Constanța, no. 25 Pepinierei Street, Valu lui Traian, Constanța Author

DOI:

https://doi.org/10.51258/AAR.2024.6.32

Keywords:

virus, diagnosis, molecular, primers

Abstract

Starting from the fact that recently the pathogen Apricot Latent Virus has been reported in many areas of the world, in order to identify a possible presence in our country, was evaluations biological material from the Constanța area. Raluca, Monica, Tomis 1 peach genotypes were evaluated both by visual observations and by laboratory tested following the molecular RT-PCR method, using 5’- GGAATAGAGCCCCAAGAAG-3’ and 5’-AGCAAGGTAAACGCCAAC-3’primers. The extraction was carried out with the help of the ”ISOLATE II Plant RNA/DNA Kit”. Evaluation of the PCR product was performed using horizontal electrophoresis on a 2% agarose gel (Cleaver), 1X TBE buffer and staining with RedSafe Nucleic Acid Staining. After testing, the diagnosis was positive for the Monica variety.

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References

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Published

01-08-2024

Issue

Vol. 6 (2024)

Section

Horticulture, Forestry and Agricultural Economics

License

Copyright (c) 2024 Acta Agricola Romanica

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

(1)
Plopa, C.; Iancu, A. F.; Gavăt, C. DETECTION OF APRICOT LATENT VIRUS TO PEACH IN CONSTANTA AREA. AAR 2024, 6, 277-281. https://doi.org/10.51258/AAR.2024.6.32.