REGENERATION POTENTIAL AND QUALITY OF REGENERANTS THROUGH ORGANOGENESIS OF PLUM AND CHERRY VARIETIES
DOI:
https://doi.org/10.51258/AAR.2025.7.27Keywords:
organogenesis, in vitro, cotyledon, plum, cherryAbstract
Screening for adventitious regeneration ability remains an important step when in vitro techniques are considered useful for clonal propagation or genetic improvement of fruit species. Studies conducted highlight the difficulties in regenerating shoots in vitro and emphasize the importance of selecting appropriate strategies for identifying and isolating transgenic cells. The investigation targeted the evaluation of direct or indirect regeneration ability from cotyledon explants of some plum and cherry cultivars and the assessment of the quality of regenerated plants (growth and vigor). Significant differences in the genetic potential for organogenesis from in vitro cultured immature cotyledons were found in both species. The overall results showed that the plum cotyledons, irrespective of culture medium and pretreatment are generally more responsive than the cherry cotyledons. The frequency of cotyledons forming shoots on the most adequate culture medium exceeded 50% in seven out of nine investigated plum cultivars. At cherry cultivars, frequency of shoot regeneration ranged from 8.3% at Van to 38.4% at Rivan. In case of cultivars which prove a low ability of shoot regeneration from their in vitro cultured immature cotyledons, wounding of cotyledons and the use of TDZ could allow higher frequencies of explants regenerating shoots, as well as a higher number of shoots formed per responsive cotyledons.Downloads
References
Adya Ram, Dennis Thomas T., Advances in Direct and Indirect Organogenesis in Forest Trees: A Review, 2024, In book: Biotechnological Approaches for Sustaining Forest Trees and Their Products. DOI: 10.1007/978-981-97-4363-6_2
Awais K. and Schuyler K., Breeding and genetics of disease resistance in temperate fruit trees: challenges and new opportunities, 2022, Theor. Appl. Genet. 135(11):3961-3985. doi: 10.1007/s00122-022-04093-0
Bini G., Bellini E., Premiers resultats de la technique de l`embryoculture applique a l`amelioration genetique du prunier (cv. Ruth Gerstetter), 1972, Acta Horticulturae 48:51-58
Canli F.A., L. Tian L., In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars, 2008, Scientia Horticulturae 116(1): 34 - 40. DOI:10.1016/j.scienta.2007.10.023
Duncan D. B., Multiple range and multiple F tests, 1955, Biometrics. 11 (1): 1–42. Doi: 10.2307/3001478. JSTOR 3001478
Escalettes V., Dosba F., In vitro adventitious shoot regeneration from leaves of Prunus spp., 1993, Plant sci. 90:201-209
FAO. The Role of Biotechnology in Exploring and Protecting Agricultural Genetic Resources, 2004, https://www.fao.org/4/a0399e/A0399E09.htm
Gribaudo, I., and Franks, T., Regeneration and transformation of fruit trees: Problems and possibilities, 2011, In A. M. Dandekar (Ed.), Transgenic Plants: Methods and Protocols (pp. 215–231), Humana Press. [https://doi.org/10.1007/978-1-61737-998-7_16](https://doi.org/10.1007/978-1-61737-998-7_16
Hammerschlag, F. A., Advances in peach, plum, and cherry biotechnology, 2002, In Vitro Cellular & Developmental Biology - Plant, 38, 560–569, doi.org/10.1079/IVP2002357
Lane W.D., Cosasio F., Adventitious shoots from cotyledons of immature cherry and apricot embryos, 1986, Can. J. Plant Sci. 66:953-959
Mante S., Scorza R., Cordts J.M., Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus, 1989, Plant Cell, Tiss. Org. Cult. 19: 1-11
Mante S., Morgens P.H., Scorza R., Cordts J.M., Callahan A.M., Agrobacterium – mediated transformation of plum (Prunus domestica L.) hypocotyl slices and regeneration of transgenic plants, 1991, Bio/Technology 9:853-857
Murashige T., Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, 1962, Physiol. Plant.15:479-497
Pence, V. C., Chaudhury, R., Luo, Y. and Xu, J., Plant biotechnology tools for conservation of genetic resources, 2022, Frontiers in Plant Science, 13, 985922, https://doi.org/10.3389/fpls.2022.985922](https://doi.org/10.3389/fpls.2022.985922
Popescu A.N., Isac V., Plant regeneration by direct and indirect organogenesis in plum (Prunus domestica L.), 1998, Inginerie Genetica si Biotehnologii Moderne, Tipografia Centrala, Khishinev
Popescu A.N., Militaru M., Cercetari privind controlul modului de regenerare in vitro de plante la prun (Prunus domestica L.), 2002, Lucrarile stiintifice ale ICDP Maracineni, Arges, Vol. XXI.:119-126
Pooler M.R., Scorza R., Plant regeneration of three rootstock cultivars from cotyledons of mature stored seeds, 1996, Hort. Science 31(6):43-47
Sarasan, V., Cripps, R., Ramsay, M. M., Atherton, C., McMichen, M., Prendergast, G., & Rowntree, J. K., In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity, 2022, Plants, 11(4), 503
Schmidt H., Kardel-Meisner U., Regeneration of adventitious shoots in vitro in cherries. III. Formation of adventitious shoots from cotyledons of sweet cherry cultivars, 1992, Gartenbauwissenschaft 57(6): 267-270
Schmidt H., Ketzel A., Regeneration of adventitious shoots in vitro in cherries. IV. Adventitious shoots regeneration of cotyledons and embryo rescue as tools in cherry breeding, 1993, Gartenbauwissenschaft 58(2): 64-67
Shubham J., Shikha J., Future prospects of biotechnology in fruit production, 2024, in book: Fruits and biotechnology, Publisher: AK international. https://www.researchgate.net/publication/385071229
Silvestri C., Mercado J.Á., Palomo-Ríos E.,Editorial: Current status of fruit tree improvement through biotechnology, 2024, Front. Plant Sci. 15:1472444. doi: 10.3389/fpls.2024.1472444
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