APPLIED BIOTECHNOLOGIES. SOLANUM LYCOPERSICUM ANTHER CULTURE

Authors

  • Andreea Elena Manolescu Alexandrescu National Research and Development Institute for Biotechnology in Horticulture - Stefanesti, Arges, Romania; Doctoral School of Plant and Animal Resources Engineering, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania; Department of Horticulture and Food Science, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania Author
  • Anamaria Mirabela Dumitru National Research and Development Institute for Biotechnology in Horticulture - Stefanesti, Arges, Romania; Doctoral School of Plant and Animal Resources Engineering, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania; Department of Horticulture and Food Science, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania Author
  • Dorin Ioan Sumedrea National Research and Development Institute for Biotechnology in Horticulture - Stefanesti, Arges, Romania; Doctoral School of Plant and Animal Resources Engineering, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania; Department of Horticulture and Food Science, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania Author
  • Sina Niculina Cosmulescu National Research and Development Institute for Biotechnology in Horticulture - Stefanesti, Arges, Romania; Doctoral School of Plant and Animal Resources Engineering, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania; Department of Horticulture and Food Science, Faculty of Horticulture, University of Craiova, A.I. Cuza Street, no. 13, 200585 Craiova, Romania Author

DOI:

https://doi.org/10.51258/AAR.2025.7.28

Keywords:

androgenesis, morphological evaluation, genetic variability, Solanum lycopersicum

Abstract

Anther culture is an efficient biotechnological method for obtaining haploid and double haploid plants by in vitro androgenesis. The technique involves the isolation of microspores from anthers and their regeneration under controlled laboratory conditions, followed by ex vitro acclimatization of the plant. This method significantly contributes to plant improvement by reducing the time required to develop new genetically stable genotypes. Evaluation of morphological traits and genetic analysis using molecular markers confirmed the haploid origin of the regenerated plants and their genetic diversity. The objectives of this study were to test the in vitro regeneration capacity of anthers in five tomato varieties obtained at INCDBH Ștefanesti, evaluate the phenotypic characteristics of the regenerated plants, and perform genetic analysis using Simple Sequence Repeat (SSR) markers to identify possible somaclonal variations resulting from the indirect organogenesis process. Successfully regenerated plants were obtained from only two of the five tested varieties, respectively 'Costate 21' and 'Argeș 20', and analysis with SSR markers revealed genetic differences between the regenerants and the donor plants, confirming that the regeneration process can generate genetic variability.

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Published

01-08-2025

Issue

Vol. 7 (2025)

Section

Horticulture, Forestry and Agricultural Economics

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Copyright (c) 2025 Acta Agricola Romanica

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

(1)
Manolescu Alexandrescu, A. E.; Dumitru, A. M.; Sumedrea, D. I.; Cosmulescu, S. N. APPLIED BIOTECHNOLOGIES. SOLANUM LYCOPERSICUM ANTHER CULTURE. AAR 2025, 7, 247-255. https://doi.org/10.51258/AAR.2025.7.28.