THE EXTENT OF SYSTEMIC IMMUNOLOGICAL CHANGES DEPEND ON HIPERSENSITIVITY LEVEL IN BOVINE TESTED FOR TUBERCULOSIS

Authors

  • A. Vasiu University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • M. Spînu University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • E. Pall University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • C.D. Șandru University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • M. Rusu University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • D. Olah University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author
  • Gh. Duca University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mǎnǎştur Street, Cluj-Napoca, Romania, Postal Code: 400372, Christian Mountainology Research – Development Institute – Sibiu, XIII Street No. 147, Christian Village, Sibiu Country Author

DOI:

https://doi.org/10.1199/1rywm753

Keywords:

tuberculosis, skin test, bovine, phagocytosis, blast transformation, immune complexes, total immune globulins

Abstract

Tuberculosis (TB), a multi-host zoonotic disease, still represents a great concern for One Health in many countries worldwide. The research, carried out on bovine from a TB positive farm (n=47, divided in three groups: positive, uncertain and negative by the skin test), where an incomplet stamping out programme was implemented, aimed at monitoring the in vitro changes of systemic immunity in relation to the skin test results. Blood was sampled twice, before and at reading of the skin test, and subjected to carbon particle inclusion and blast transformation tests to evaluate cell mediated responses to mitogens, vegetal extracts and bovine/avian PPD. Circulating immune complexes (CIC) and immune globulins (Ig) were quantified by PEG 4.2% and ZnSO424%o preciptation methods, respectively. The results indicated the lowest values for phagocyatosis in the positive animals, while the blast transformation was most stimulated towards avian and bovine PPD (93.51±24.26% and 91.21±18.04%, respectively). An increased humoral immune activity was also recorded in the positive group, which showed the highest CIC (0.281 ± 0.017ODU) and Ig (0.182 ± 0.004 ODU) values. A positive, statistically relevant (p<0.001) correlation between the phagocytic capacity, CIC, Ig and the cutaneous reactivity was poited out. All tests indicated extensive changes in innate and adaptive immunity, suggesting their use, although not golden standard could be beneficial in surveillance of TB immunity in positive herds.

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Published

2023-08-01

Issue

Vol. 5 No. 3 (2023): Animal Sciences and Veterinary Medicine

Section

Articles