BOOSTING TOMATOES NATIVE RESISTANCE TO ABIOTIC STRESS THROUGH EXOGENOUS SALICYLIC ACID UTILIZATION IN TISSUE CULTURE TECHNIQUES “IN VITRO”
DOI:
https://doi.org/10.51258/AAR.2024.6.24Keywords:
salicylic acid, in vitro, sustainable, vegetable, breedingAbstract
Tomato (Solanum lycopersicum Mill.) is a vital crop facing increased abiotic stress due to climate change. Drought, salinity, and temperature fluctuations can severely impact growth and yield. This study examines using salicylic acid (SA) in tissue culture to enhance tomato plants' resistance to abiotic stress "in vitro." Tomato plants were cultured in controlled "in vitro" conditions with varying SA concentrations (1 mM/l, 0.5 mM/l, and 0.1 mM/l) in MS 1962 media. We assessed seed germination, shoot initiation, root development, and physiological parameters (phenolic and chlorophyll content). Results show that SA application significantly improves stress tolerance, enhancing plant growth and chlorophyll levels while reducing oxidative stress. SA-treated plants upregulate stress-responsive genes, activating defense mechanisms against abiotic stress. Using SA in tissue culture shows promise for sustainable agriculture by boosting tomato plants' native defenses against environmental challenges, mitigating yield losses, and enhancing food security and agricultural sustainability. This approach offers insights into developing strategies to improve crop resilience amid changing environmental conditions.Downloads
References
AHMED, H.S. et al.; ‘Salicylic acid increases flavonolignans accumulation in the fruits of hydroponically cultured Silybum marianum’; 2020; Saudi Pharmaceutical Journal, 28(5), pp. 593–598
BAI, Y. et al.; ‘Tomato disease resistances in the post-genomics era’; 2016; in: V International Symposium on Tomato Diseases: Perspectives and Future Directions in Tomato Protection 1207, pp. 1–18
GHOSH, A., Igamberdiev, A.U. and Debnath, S.C.; ‘Tissue culture-induced DNA methylation in crop plants: A review’; 2021; Molecular biology reports, 48(1), pp. 823–841
GIRI, A. and Giri, C.C.; ‘Recent Trends in Elicitation and Secondary Metabolic Pathway Analysis in Medicinal Plants: An Integrated Transcriptomics and Proteomics Approach’; 2022; in: Medicinal and Aromatic Plants of India Vol. 1. Springer, pp. 403–430
JOSEPH, B., Jini, D. and Sujatha, S.; ‘Insight into the role of exogenous salicylic acid on’; 2010; Asian Journal of Crop Science, 2(4), pp. 226–235
KHAN, W., Prithiviraj, B. and Smith, D.L.; ‘Photosynthetic responses of corn and soybean to foliar application of salicylates’; 2003; Journal of plant physiology, 160(5), pp. 485–492
KHODARY, S.; ‘Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants’; 2004; Int. J. Agric. Biol, 6(1), pp. 5–8
KUMAR, A. et al.; ‘Tomato (Solanum Lycopersicon)’, Antioxidants in vegetables and nuts-Properties and health benefits; (2020); pp. 191–207
KUMAR, M. et al.; ‘Tomato (Solanum lycopersicum L.) seed: A review on bioactives and biomedical activities’; 2021; Biomedicine & Pharmacotherapy, 142, p. 112018
LEFEVERE, H., Bauters, L. and Gheysen, G.; ‘Salicylic acid biosynthesis in plants’; 2020; Frontiers in plant science, 11, p. 521987
Lichtenthaler, H.K.; ‘[34] Chlorophylls and carotenoids: pigments of photosynthetic biomembranes’; 1987; in: Methods in enzymology. Elsevier, pp. 350–382
NGULLIE, C., Tank, R. and Bhanderi, D.; ‘Effect of salicylic acid and humic acid on flowering, fruiting, yield and quality of mango (Mangifera indica L.) cv. KESAR’; 2014
SINGH, V.K. et al.; ‘Manipulating epigenetic diversity in crop plants: Techniques, challenges and opportunities’; 2023; Biochimica et Biophysica Acta (BBA)-General Subjects, p. 130544
SUPRASANNA, P.; ‘Plant abiotic stress tolerance: Insights into resilience build-up’; 2020; Journal of biosciences, 45(1), p. 120
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Acta Agricola Romanica
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
